1 February 2011 Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering
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Abstract
We demonstrate two different coherent anti-Stokes Raman scattering (CARS) microscopy and microspectroscopy methods based on the spectral focusing mechanism. The first method uses strongly chirped broadband pulses from a single Ti:sapphire laser and generates CARS signals at the fingerprint region. Fast modulation of the time delay between the pump and Stokes laser pulses coupled with lock-in signal detection significantly reduces the nonresonant background and produces Raman-like CARS signals with a spectral resolution of 20 cm−1. The second method generates CARS signals in the CH (carbon-hydrogen) stretching region with IR supercontinuum pulses from a photonic crystal fiber. The spectral resolution of 30 cm−1 is achieved. Maximum entropy method is used to retrieve a Raman-equivalent CARS spectrum from lipid membranes. Chemical imaging and microspectroscopy are demonstrated with various samples.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
Bi-Chang Chen, Jiha Sung, Xiaoxi Wu, Sang-Hyun Lim, "Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering," Journal of Biomedical Optics 16(2), 021112 (1 February 2011). https://doi.org/10.1117/1.3533315 . Submission:
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