1 February 2011 Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis
Author Affiliations +
Contemporary brain tumor research focuses on two challenges: First, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. Second, for prognostication the tumor has to be removed as completely as possible. Nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. However, it is neither applicable in vivo, nor does it allow for precise tumor typing in those cases when only nonrepresentative specimens are procured. Infrared and Raman spectroscopy allow for very precise cancer analysis due to their molecular specificity, while nonlinear microscopy is a suitable tool for rapid imaging of large tissue sections. Here, unstained samples from the brain of a domestic pig have been investigated by a multimodal nonlinear imaging approach combining coherent anti-Stokes Raman scattering, second harmonic generation, and two photon excited fluorescence microscopy. Furthermore, a brain tumor specimen was additionally analyzed by linear Raman and Fourier transform infrared imaging for a detailed assessment of the tissue types that is required for classification and to validate the multimodal imaging approach. Hence label-free vibrational microspectroscopic imaging is a promising tool for fast and precise in vivo diagnostics of brain tumors.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
Tobias Meyer, Tobias Meyer, Norbert Bergner, Norbert Bergner, Christoph Krafft, Christoph Krafft, Denis Akimov, Denis Akimov, Benjamin Dietzek, Benjamin Dietzek, Jürgen Popp, Jürgen Popp, Christiane Bielecki, Christiane Bielecki, Bernd F. M. Romeike, Bernd F. M. Romeike, Rupert Reichart, Rupert Reichart, Rolf Kalff, Rolf Kalff, } "Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis," Journal of Biomedical Optics 16(2), 021113 (1 February 2011). https://doi.org/10.1117/1.3533268 . Submission:

Back to Top