1 February 2011 Label-free imaging of biomolecules in food products using stimulated Raman microscopy
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The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in multiphasic systems. SRS combines the label-free molecular specificity of vibrational spectroscopy with an enhanced sensitivity due to coherent excitation of molecular vibrations. Compared to previous imaging studies using coherent anti-Stokes Raman scattering microscopy, the main advantage of SRS microscopy is the absence of the unwanted nonresonant background, which translates into a superior sensitivity and undistorted vibrational spectra. We compare spectra of complex materials obtained with stimulated Raman scattering and spontaneous Raman scattering in the crowded fingerprint region. We find that, as expected, there is excellent correspondence and that the SRS spectra are free from interference from background fluorescence. In addition, we show high-resolution imaging of the distributions of selected biomolecules, such as lipids and proteins, in food products with SRS microscopy.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
Maarten B. J. Roeffaers, Maarten B. J. Roeffaers, Xu Zhang, Xu Zhang, Christian W. Freudiger, Christian W. Freudiger, Brian G. Saar, Brian G. Saar, X. Sunney Xie, X. Sunney Xie, Marjolein van Ruijven, Marjolein van Ruijven, Gerard van Dalen, Gerard van Dalen, Chunhong Xiao, Chunhong Xiao, } "Label-free imaging of biomolecules in food products using stimulated Raman microscopy," Journal of Biomedical Optics 16(2), 021118 (1 February 2011). https://doi.org/10.1117/1.3516591 . Submission:

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