Simplified approach for quantitative digital holographic phase contrast imaging of living cells

Björn Kemper; Angelika Vollmer; Gert von Bally; Christina E. Rommel; Jürgen Schnekenburger

doi:10.1117/1.3540674

1 February 2011 Simplified approach for quantitative digital holographic phase contrast imaging of living cells

Björn Kemper, Angelika Vollmer, Gert von Bally, Christina E. Rommel, Jürgen Schnekenburger

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Journal of Biomedical Optics, Vol. 16, Issue 2, 026014 (February 2011). https://doi.org/10.1117/1.3540674

Abstract

Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in a low phase stability and the demand for a precise adjustment of the intensity ratio between object and reference wave. To overcome these problems, the performance of a Michelson interferometer approach for digital holographic microscopy was analyzed that avoids a separately generated reference wave by superposition of different image areas. It is shown that this simplified arrangement yields improved phase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstrate the capability of the method for reliable quantitative phase contrast imaging.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Björn Kemper, Angelika Vollmer, Gert von Bally, Christina E. Rommel, and Jürgen Schnekenburger "Simplified approach for quantitative digital holographic phase contrast imaging of living cells," Journal of Biomedical Optics 16(2), 026014 (1 February 2011). https://doi.org/10.1117/1.3540674

Published: 1 February 2011

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KEYWORDS

Digital holography

Phase contrast

Holograms

3D image reconstruction

Mirrors

Digital imaging

Holography

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