Spatially correlated microthermography maps threshold temperature in laser-induced damage

Michael L. Denton; Gary D. Noojin; Michael S. Foltz; Clifton D. Clark III; Larry E. Estlack; Benjamin A. Rockwell; Robert J. Thomas

doi:10.1117/1.3548881

1 March 2011 Spatially correlated microthermography maps threshold temperature in laser-induced damage

Michael L. Denton, Gary D. Noojin, Michael S. Foltz, Clifton D. Clark III, Larry E. Estlack, Benjamin A. Rockwell, Robert J. Thomas

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Journal of Biomedical Optics, Vol. 16, Issue 3, 036003 (March 2011). https://doi.org/10.1117/1.3548881

Abstract

We measured threshold temperatures for cell death resulting from short (0.1-1.0 s) 514-nm laser exposures using an in vitro retinal model. Real-time thermal imaging at sub-cellular resolution provides temperature information that is spatially correlated with cells at the boundary of cell death, as indicate by post-exposure fluorescence images. Our measurements indicate markedly similar temperatures, not only around individual boundaries (single exposure), but among all exposures of the same duration in a laser irradiance-independent fashion. Two different methods yield similar threshold temperatures with low variance. Considering the experimental uncertainties associated with the thermal camera, an average peak temperature of 53 ± 2 °C is found for laser exposures of 0.1, 0.25, and 1.0 s. Additionally, we find a linear relationship between laser exposure duration and time-averaged integrated temperature. The mean thermal profiles for cells at the boundary of death were assessed using the Arrhenius rate law using parameter sets (frequency factor and energy of activation) found in three different articles.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Michael L. Denton, Gary D. Noojin, Michael S. Foltz, Clifton D. Clark III, Larry E. Estlack, Benjamin A. Rockwell, and Robert J. Thomas "Spatially correlated microthermography maps threshold temperature in laser-induced damage," Journal of Biomedical Optics 16(3), 036003 (1 March 2011). https://doi.org/10.1117/1.3548881

Published: 1 March 2011

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