1 March 2011 Photoacoustic tomography of ex vivo mouse hearts with myocardial infarction
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Abstract
In the present study, we evaluated the applicability of ex vivo photoacoustic imaging (PAI) on small animal organs. We used photoacoustic tomography (PAT) to visualize infarcted areas within murine hearts and compared these data to other imaging techniques [magnetic resonance imaging (MRI), micro-computed tomography] and histological slices. In order to induce ischemia, an in vivo ligation of the left anterior descending artery was performed on nine wild-type mice. After varying survival periods, the hearts were excised and fixed in formaldehyde. Samples were illuminated with nanosecond laser pulses delivered by a Nd:YAG pumped optical parametric oscillator. Ultrasound detection was achieved using a Mach-Zehnder interferometer (MZI) working as an integrating line detector. The voxel data were computed using a Fourier-domain based reconstruction algorithm, followed by inverse Radon transforms. The results clearly showed the capability of PAI to visualize myocardial infarction and to produce three-dimensional images with a spatial resolution of approximately 120 μm. Regions of affected muscle tissue in PAI corresponded well with the results of MRI and histology. Photoacoustic tomography utilizing a MZI for ultrasound detection allows for imaging of small tissue samples. Due to its high spatial resolution, good soft tissue contrast and comparatively low cost, PAT offers great potentials for imaging.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
Markus Holotta, Christian Kremser, Pavle Torbica, Gerald Degenhart, Regina Esterhammer, Werner Jaschke, Harald Grossauer, Jakob Völkl, Robert Nuster, Günther Paltauf, "Photoacoustic tomography of ex vivo mouse hearts with myocardial infarction," Journal of Biomedical Optics 16(3), 036007 (1 March 2011). https://doi.org/10.1117/1.3556720 . Submission:
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