1 April 2011 Long-term imaging of mouse embryos using adaptive harmonic generation microscopy
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J. of Biomedical Optics, 16(4), 046018 (2011). doi:10.1117/1.3569614
We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5-5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, such as lipid droplets, nucleoli, and plasma membranes. The embryos are found to develop normally during one-day-long discontinuous HG imaging, permitting the observation of several dynamic events, such as morula compaction and blastocyst formation.
Anisha Thayil, Alexander Jesacher, Tony Wilson, Martin Booth, Tomoko Watanabe, Shankar Srinivas, "Long-term imaging of mouse embryos using adaptive harmonic generation microscopy," Journal of Biomedical Optics 16(4), 046018 (1 April 2011). https://doi.org/10.1117/1.3569614

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