1 April 2011 Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements
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Abstract
In this study, we demonstrate the potential of employing third harmonic generation (THG) imaging microscopy measurements for cell tracking studies in live Caenorhabditis elegans (C. elegans) embryos. A 1028-nm femtosecond laser was used for the excitation of unstained C. elegans samples. Different C. elegans embryonic stages (from two-cell to threefold) were imaged. Live biological specimens were irradiated for prolonged periods of time (up to 7 h), testifying to the nondestructive nature of this nonlinear imaging technique. Thus, THG image contrast modality is a powerful diagnostic tool for probing in vivo cell division during early embryogenesis.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
George J. Tserevelakis, George Filippidis, Costas Fotakis, Evgenia V. Megalou, Nektarios Tavemarakis, "Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements," Journal of Biomedical Optics 16(4), 046019 (1 April 2011). https://doi.org/10.1117/1.3569615 . Submission:
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