1 April 2011 Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements
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Abstract
In this study, we demonstrate the potential of employing third harmonic generation (THG) imaging microscopy measurements for cell tracking studies in live Caenorhabditis elegans (C. elegans) embryos. A 1028-nm femtosecond laser was used for the excitation of unstained C. elegans samples. Different C. elegans embryonic stages (from two-cell to threefold) were imaged. Live biological specimens were irradiated for prolonged periods of time (up to 7 h), testifying to the nondestructive nature of this nonlinear imaging technique. Thus, THG image contrast modality is a powerful diagnostic tool for probing in vivo cell division during early embryogenesis.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
George J. Tserevelakis, George J. Tserevelakis, George Filippidis, George Filippidis, Costas Fotakis, Costas Fotakis, Evgenia V. Megalou, Evgenia V. Megalou, Nektarios Tavemarakis, Nektarios Tavemarakis, } "Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements," Journal of Biomedical Optics 16(4), 046019 (1 April 2011). https://doi.org/10.1117/1.3569615 . Submission:
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