1 May 2011 Spectroscopic signature of mouse embryonic stem cell-derived hepatocytes using synchrotron Fourier transform infrared microspectroscopy
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Abstract
Stem cell-based therapy for liver regeneration has been proposed to overcome the persistent shortage in the supply of suitable donor organs. A requirement for this to succeed is to find a rapid method to detect functional hepatocytes, differentiated from embryonic stem cells. We propose Fourier transform infrared (FTIR) microspectroscopy as a versatile method to identify the early and last stages of the differentiation process leading to the formation of hepatocytes. Using synchrotron-FTIR microspectroscopy, the means of identifying hepatocytes at the single-cell level is possible and explored. Principal component analysis and subsequent partial least-squares (PLS) discriminant analysis is applied to distinguish endoderm induction from hepatic progenitor cells and matured hepatocyte-like cells. The data are well modeled by PLS with endoderm induction, hepatic progenitor cells, and mature hepatocyte-like cells able to be discriminated with very high sensitivity and specificity. This method provides a practical tool to monitor endoderm induction and has the potential to be applied for quality control of cell differentiation leading to hepatocyte formation.
© (2011) Society of Photo-Optical Instrumentation Engineers (SPIE)
Kanjana Thumanu, Kanjana Thumanu, Waraporn Tanthanuch, Waraporn Tanthanuch, Danna Ye, Danna Ye, Anawat Sangmalee, Anawat Sangmalee, Rangsun Parnpai, Rangsun Parnpai, Chanchao Lorthongpanich, Chanchao Lorthongpanich, Philip Heraud, Philip Heraud, } "Spectroscopic signature of mouse embryonic stem cell-derived hepatocytes using synchrotron Fourier transform infrared microspectroscopy," Journal of Biomedical Optics 16(5), 057005 (1 May 2011). https://doi.org/10.1117/1.3580253 . Submission:
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