Automatic cytometric device using multiple wavelength excitations

Nelly Rongeat; Sylvain Ledroit; Laurence Chauvet; Didier Cremien; Alexandra Urankar; Philippe Nerin; Vincent Couderc

doi:10.1117/1.3582162

1 May 2011 Automatic cytometric device using multiple wavelength excitations

Nelly Rongeat, Sylvain Ledroit, Laurence Chauvet, Didier Cremien, Alexandra Urankar, Philippe Nerin, Vincent Couderc

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Journal of Biomedical Optics, Vol. 16, Issue 5, 057006 (May 2011). https://doi.org/10.1117/1.3582162

Abstract

Precise identification of eosinophils, basophils, and specific subpopulations of blood cells (B lymphocytes) in an unconventional automatic hematology analyzer is demonstrated. Our specific apparatus mixes two excitation radiations by means of an acousto-optics tunable filter to properly control fluorescence emission of phycoerythrin cyanin 5 (PC5) conjugated to antibodies (anti-CD20 or anti-CRTH2) and Thiazole Orange. This way our analyzer combining techniques of hematology analysis and flow cytometry based on multiple fluorescence detection, drastically improves the signal to noise ratio and decreases the spectral overlaps impact coming from multiple fluorescence emissions.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Nelly Rongeat, Sylvain Ledroit, Laurence Chauvet, Didier Cremien, Alexandra Urankar, Philippe Nerin, and Vincent Couderc "Automatic cytometric device using multiple wavelength excitations," Journal of Biomedical Optics 16(5), 057006 (1 May 2011). https://doi.org/10.1117/1.3582162

Published: 1 May 2011

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