CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 17 > Issue 10 > Article
Open Access
15 October 2012 Photo-sensitive hydrogels for three-dimensional laser microfabrication in the presence of whole organisms
Jan Torgersen, Aleksandr Ovsianikov, Vladimir Mironov, Niklas Pucher, Xiaohua Qin, Zhiquan Li, Klaus Cicha, Thomas Machacek, Robert Liska, Verena Jantsch, Jürgen Stampfl
Author Affiliations +
Journal of Biomedical Optics, Vol. 17, Issue 10, 105008 (October 2012). https://doi.org/10.1117/1.JBO.17.10.105008
Abstract
Hydrogels are polymeric materials with water contents similar to that of soft tissues. Due to their biomimetic properties, they have been extensively used in various biomedical applications including cell encapsulation for tissue engineering. The utilization of photopolymers provides a possibility for the temporal and spatial controlling of hydrogel cross-links. We produced three-dimensional (3-D) hydrogel scaffolds by means of the two-photon polymerization (2PP) technique. Using a highly efficient water-soluble initiator, photopolymers with up to 80 wt.% water were processed with high precision and reproducibility at a writing speed of 10  mm/s . The biocompatibility of the applied materials was verified using Caenorhabditis elegans as living test organisms. Furthermore, these living organisms were successfully embedded within a 200×200×35 μm 3 hydrogel scaffold. As most biologic tissues exhibit a window of transparency at the wavelength of the applied femtosecond laser, it is suggested that 2PP is promising for an in situ approach. Our results demonstrate the feasibility of and potential for bio-fabricating 3-D tissue constructs in the micrometre-range via near-infrared lasers in direct contact with a living organism.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
Citation Download Citation
Jan Torgersen, Aleksandr Ovsianikov, Vladimir Mironov, Niklas Pucher, Xiaohua Qin, Zhiquan Li, Klaus Cicha, Thomas Machacek, Robert Liska, Verena Jantsch, and Jürgen Stampfl "Photo-sensitive hydrogels for three-dimensional laser microfabrication in the presence of whole organisms," Journal of Biomedical Optics 17(10), 105008 (15 October 2012). https://doi.org/10.1117/1.JBO.17.10.105008
Published: 15 October 2012
JOURNAL ARTICLE
 10 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 124 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Two photon polymerization
Organisms
Tissues
Polymers
Microfabrication
Near infrared
Computer aided design
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top