1 November 2012 In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma
Author Affiliations +
Abstract
Tissue diagnostic features generated by a bimodal technique integrating scanning time-resolved fluorescence spectroscopy (TRFS) and ultrasonic backscatter microscopy (UBM) are investigated in an in vivo hamster oral carcinoma model. Tissue fluorescence is excited by a pulsed nitrogen laser and spectrally and temporally resolved using a set of filters/dichroic mirrors and a fast digitizer, respectively. A 41-MHz focused transducer (37-μm axial, 65-μmm lateral resolution) is used for UBM scanning. Representative lesions of the different stages of carcinogenesis show that fluorescence characteristics complement ultrasonic features, and both correlate with histological findings. These results demonstrate that TRFS-UBM provide a wealth of co-registered, complementary data concerning tissue composition and structure as it relates to disease status. The direct co-registration of the TRFS data (sensitive to surface molecular changes) with the UBM data (sensitive to cross-sectional structural changes and depth of tumor invasion) is expected to play an important role in pre-operative diagnosis and intra-operative determination of tumor margins.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yang Sun, Yang Sun, Hongtao Xie, Hongtao Xie, Jing Liu, Jing Liu, Matthew Lam, Matthew Lam, Abhijit J. Chaudhari, Abhijit J. Chaudhari, Feifei Zhou, Feifei Zhou, Julien Bec, Julien Bec, Diego R. Yankelevich, Diego R. Yankelevich, Allison Dobbie, Allison Dobbie, Steven P. Tinling, Steven P. Tinling, Regina Gandour-Edwards, Regina Gandour-Edwards, Wayne L. Monsky, Wayne L. Monsky, Gregory D. Farwell, Gregory D. Farwell, Laura Marcu, Laura Marcu, } "In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma," Journal of Biomedical Optics 17(11), 116003 (1 November 2012). https://doi.org/10.1117/1.JBO.17.11.116003 . Submission:
JOURNAL ARTICLE
11 PAGES


SHARE
Back to Top