1 November 2012 Spectral imaging technique for retinal perfusion detection using confocal scanning laser ophthalmoscopy
Author Affiliations +
J. of Biomedical Optics, 17(11), 116005 (2012). doi:10.1117/1.JBO.17.11.116005
To evaluate retinal perfusion in the human eye, a dual-wavelength confocal scanning laser ophthalmoscope (cSLO) was developed that provides spectral imaging of the fundus using a combination of red (670 nm) and near-infrared (810 nm) wavelengths. The image of the ocular fundus was analyzed to find out if quantitative measurements of the reflectivity of tissue permit assessment of the oxygen perfusion of tissue. We explored problems that affect the reproducibility of patient measurements such as non-uniformity errors on the image. For the first time, an image processing technique was designed and used to minimize the errors of oxygen saturation measurements by illumination correction in retina wide field by increasing SNR. Retinal images were taken from healthy and diabetic retinopathy eyes using the cSLO with a confocal aperture of 100 μm. The ratio image (RI) of red/IR, as oxygen saturation (SO2) index, was calculated for normal eyes. The image correction technique improved the reproducibility of the measurements. Average RI intensity variation of healthy retina tissue was determined within a range of about 5.5%. The capability of the new technique to discriminate oxygenation levels of retinal artery and vein was successfully demonstrated and showed good promise in the diagnosis of the perfused retina.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Seyed Hossein Rasta, Ayyakkannu Manivannan, Peter F. Sharp, "Spectral imaging technique for retinal perfusion detection using confocal scanning laser ophthalmoscopy," Journal of Biomedical Optics 17(11), 116005 (1 November 2012). https://doi.org/10.1117/1.JBO.17.11.116005




Scanning laser ophthalmoscopy

Confocal microscopy



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