3 December 2012 Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy
Author Affiliations +
J. of Biomedical Optics, 17(12), 126008 (2012). doi:10.1117/1.JBO.17.12.126008
We present Localizer, a freely available and open source software package that implements the computational data processing inherent to several types of superresolution fluorescence imaging, such as localization (PALM/STORM/GSDIM) and fluctuation imaging (SOFI/pcSOFI). Localizer delivers high accuracy and performance and comes with a fully featured and easy-to-use graphical user interface but is also designed to be integrated in higher-level analysis environments. Due to its modular design, Localizer can be readily extended with new algorithms as they become available, while maintaining the same interface and performance. We provide front-ends for running Localizer from Igor Pro, Matlab, or as a stand-alone program. We show that Localizer performs favorably when compared with two existing superresolution packages, and to our knowledge is the only freely available implementation of SOFI/pcSOFI microscopy. By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Peter Dedecker, Sam Duwé, Robert K. Neely, Jin Zhang, "Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy," Journal of Biomedical Optics 17(12), 126008 (3 December 2012). https://doi.org/10.1117/1.JBO.17.12.126008

Super resolution


Signal to noise ratio


Data processing

Biomedical optics

Image analysis

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