12 December 2012 Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: an in vitro study of macrophages as a vector for the delivery of gold nanoshells
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Abstract
Photothermal treatment (PTT) using nanoparticles has gained attention as a promising alternative therapy for malignant tumors. One strategy for increasing the selectivity of PTT is the use of macrophages as a cellular vector for delivering nanoparticles. The aim of the present study is to examine the use of macrophages as a cellular vector for efficient PTT and determine the appropriate irradiation power and time of a near-infrared (NIR) laser using real-time phase-contrast imaging. Thermally induced injury and death of cancer cells were found to begin at 44°C to 45°C, which was achieved using the PTT effect with gold nanoshells (NS) and irradiation with a NIR laser at a power of 2 W for 5 min. The peritoneal macrophage efficiently functioned as a cellular vector for the NS, and the cancer cells surrounding the NS-loaded macrophages selectively lost their cellular viability after being irradiated with the NIR laser.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Taeseok D. Yang, Wonshik Choi, Tai Hyun Yoon, Kyoung Jin Lee, Jae-Seung Lee, Sang Hun Han, Min-Goo Lee, Hong Soon Yim, Kyung Min Choi, Min Woo Park, Kwang-Yoon Jung, Seung-Kuk Baek, "Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: an in vitro study of macrophages as a vector for the delivery of gold nanoshells," Journal of Biomedical Optics 17(12), 128003 (12 December 2012). https://doi.org/10.1117/1.JBO.17.12.128003 . Submission:
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