23 February 2012 Three-dimensional differential interference contrast microscopy using synthetic aperture imaging
Author Affiliations +
We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Moonseok Kim, Moonseok Kim, Youngwoon Choi, Youngwoon Choi, Wonshik Choi, Wonshik Choi, Christopher M. Fang-Yen, Christopher M. Fang-Yen, Yongjin Sung, Yongjin Sung, Ramachandra Rao Dasari, Ramachandra Rao Dasari, Michael S. Feld, Michael S. Feld, Kwanhyung Kim, Kwanhyung Kim, } "Three-dimensional differential interference contrast microscopy using synthetic aperture imaging," Journal of Biomedical Optics 17(2), 026003 (23 February 2012). https://doi.org/10.1117/1.JBO.17.2.026003 . Submission:

Back to Top