Hyperspectral imaging for early detection of oxygenation and perfusion changes in irradiated skin

Michael S. Chin; Brian B. Freniere; Yuan-Chyuan Lo; Jonathon H. Saleeby; Stephen P. Baker; Heather M. Strom; Ronald A. Ignotz; Janice F. Lalikos; Thomas J. Fitzgerald

doi:10.1117/1.JBO.17.2.026010

5 March 2012 Hyperspectral imaging for early detection of oxygenation and perfusion changes in irradiated skin

Michael S. Chin, Brian B. Freniere, Yuan-Chyuan Lo, Jonathon H. Saleeby, Stephen P. Baker, Heather M. Strom, Ronald A. Ignotz, Janice F. Lalikos, Thomas J. Fitzgerald

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 2, 026010 (March 2012). https://doi.org/10.1117/1.JBO.17.2.026010

Abstract

Studies examining acute oxygenation and perfusion changes in irradiated skin are limited. Hyperspectral imaging (HSI), a method of wide-field, diffuse reflectance spectroscopy, provides noninvasive, quantified measurements of cutaneous oxygenation and perfusion. This study examines whether HSI can assess acute changes in oxygenation and perfusion following irradiation. Skin on both flanks of nude mice (n = 20) was exposed to 50 Gy of beta radiation from a strontium-90 source. Hyperspectral images were obtained before irradiation and on selected days for three weeks. Skin reaction assessment was performed concurrently with HSI. Desquamative injury formed in all irradiated areas. Skin reactions were first seen on day 7, with peak formation on day 14, and resolution beginning by day 21. HSI demonstrated increased tissue oxygenation on day 1 before cutaneous changes were observed (p<0.001). Further increases over baseline were seen on day 14, but returned to baseline levels by day 21. For perfusion, similar increases were seen on days 1 and 14. Unlike tissue oxygenation, perfusion was decreased below baseline on day 21 (p<0.002). HSI allows for complete visualization and quantification of tissue oxygenation and perfusion changes in irradiated skin, and may also allow prediction of acute skin reactions based on early changes seen after irradiation.

Citation Download Citation

Michael S. Chin, Brian B. Freniere, Yuan-Chyuan Lo, Jonathon H. Saleeby, Stephen P. Baker, Heather M. Strom, Ronald A. Ignotz, Janice F. Lalikos, and Thomas J. Fitzgerald "Hyperspectral imaging for early detection of oxygenation and perfusion changes in irradiated skin," Journal of Biomedical Optics 17(2), 026010 (5 March 2012). https://doi.org/10.1117/1.JBO.17.2.026010

Published: 5 March 2012

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY