3 April 2012 Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator
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Abstract
Through a combination of a deflective phase-only diffractive spatial light modulator (SLM) and galvo scanners, an addressable multiregional and multifocal multiphoton microscope (AM-MMM) is developed. The SLM shapes an incoming mode-locked, near-infrared Ti:sapphire laser beam into multiple beamlet arrays with addressable shapes and sizes that match the regions of interest on the sample. Compared with conventional multifocal multiphoton microscope (MMM), AM-MMM achieves the effective use of the laser power with an increase of imaging rate and a decrease of photodamage without sacrifice of resolution.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yonghong Shao, Yonghong Shao, Junle Qu, Junle Qu, Xiang Peng, Xiang Peng, Hanben Niu, Hanben Niu, Wan Qin, Wan Qin, Honghai Liu, Honghai Liu, Bruce Z. Gao, Bruce Z. Gao, } "Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator," Journal of Biomedical Optics 17(3), 030505 (3 April 2012). https://doi.org/10.1117/1.JBO.17.3.030505 . Submission:
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