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20 March 2012 Approach to quantify human dermal skin aging using multiphoton laser scanning microscopy
Stefan Puschmann, Christian-Dennis Rahn, Horst Wenck, Stefan Gallinat, Frank F. Fischer
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Journal of Biomedical Optics, Vol. 17, Issue 3, 036005 (March 2012). https://doi.org/10.1117/1.JBO.17.3.036005
Abstract
Extracellular skin structures in human skin are impaired during intrinsic and extrinsic aging. Assessment of these dermal changes is conducted by subjective clinical evaluation and histological and molecular analysis. We aimed to develop a new parameter for the noninvasive quantitative determination of dermal skin alterations utilizing the high-resolution three-dimensional multiphoton laser scanning microscopy (MPLSM) technique. To quantify structural differences between chronically sun-exposed and sun-protected human skin, the respective collagen-specific second harmonic generation and the elastin-specific autofluorescence signals were recorded in young and elderly volunteers using the MPLSM technique. After image processing, the elastin-to-collagen ratio (ELCOR) was calculated. Results show that the ELCOR parameter of volar forearm skin significantly increases with age. For elderly volunteers, the ELCOR value calculated for the chronically sun-exposed temple area is significantly augmented compared to the sun-protected upper arm area. Based on the MPLSM technology, we introduce the ELCOR parameter as a new means to quantify accurately age-associated alterations in the extracellular matrix.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Stefan Puschmann, Christian-Dennis Rahn, Horst Wenck, Stefan Gallinat, and Frank F. Fischer "Approach to quantify human dermal skin aging using multiphoton laser scanning microscopy," Journal of Biomedical Optics 17(3), 036005 (20 March 2012). https://doi.org/10.1117/1.JBO.17.3.036005
Published: 20 March 2012
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KEYWORDS
Skin
In vivo imaging
Collagen
Second-harmonic generation
Optical fibers
Atrial fibrillation
Laser scanners
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