7 May 2012 Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics
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Abstract
Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Martí Duocastella, Martí Duocastella, Bo Sun, Bo Sun, Craig B. Arnold, Craig B. Arnold, } "Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics," Journal of Biomedical Optics 17(5), 050505 (7 May 2012). https://doi.org/10.1117/1.JBO.17.5.050505 . Submission:
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