5 June 2012 Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging
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Abstract
In this study, we demonstrate a method to quantify biomarker expression that uses an exogenous dual-reporter imaging approach to improve tumor signal detection. The uptake of two fluorophores, one nonspecific and one targeted to the epidermal growth factor receptor (EGFR), were imaged at 1 h in three types of xenograft tumors spanning a range of EGFR expression levels (n  =  6 in each group). Using this dual-reporter imaging methodology, tumor contrast-to-noise ratio was amplified by >6 times at 1 h postinjection and >2 times at 24 h. Furthermore, by as early as 20 min postinjection, the dual-reporter imaging signal in the tumor correlated significantly with a validated marker of receptor density (P  <  0.05, r  =  0.93). Dual-reporter imaging can improve sensitivity and specificity over conventional fluorescence imaging in applications such as fluorescence-guided surgery and directly approximates the receptor status of the tumor, a measure that could be used to inform choices of biological therapies.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Kenneth M. Tichauer, Kenneth M. Tichauer, Kimberley S. Samkoe, Kimberley S. Samkoe, Kristian J. Sexton, Kristian J. Sexton, Jason R. Gunn, Jason R. Gunn, Tayyaba Hasan, Tayyaba Hasan, Brian W. Pogue, Brian W. Pogue, } "Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging," Journal of Biomedical Optics 17(6), 066001 (5 June 2012). https://doi.org/10.1117/1.JBO.17.6.066001 . Submission: Received: 1 December 2011; Accepted: 11 April 2012
Received: 1 December 2011; Accepted: 11 April 2012; Published: 5 June 2012
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