5 June 2012 Monitoring the process of pulmonary melanoma metastasis using large area and label-free nonlinear optical microscopy
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J. of Biomedical Optics, 17(6), 066002 (2012). doi:10.1117/1.JBO.17.6.066002
Abstract
We performed large area nonlinear optical microscopy (NOM) for label-free monitoring of the process of pulmonary melanoma metastasis ex vivo with subcellular resolution in C57BL/6 mice. Multiphoton autofluorescence (MAF) and second harmonic generation (SHG) images of lung tissue are obtained in a volume of ∼ 2.2  mm×2.2  mm×30  μm. Qualitative differences in morphologic features and quantitative measurement of pathological lung tissues at different time points are characterized. We find that combined with morphological features, the quantitative parameters, such as the intensity ratio of MAF and SHG between pathological tissue and normal tissue and the MAF to SHG index versus depth clearly shows the tissue physiological changes during the process of pulmonary melanoma metastasis. Our results demonstrate that large area NOM succeeds in monitoring the process of pulmonary melanoma metastasis, which can provide a powerful tool for the research in tumor pathophysiology and therapy evaluation.
Daozhu Hua, Shuhong Qi, Hui Li, Zhihong Zhang, Ling Fu, "Monitoring the process of pulmonary melanoma metastasis using large area and label-free nonlinear optical microscopy," Journal of Biomedical Optics 17(6), 066002 (5 June 2012). http://dx.doi.org/10.1117/1.JBO.17.6.066002
Submission: Received ; Accepted
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KEYWORDS
Tissues

Second-harmonic generation

Melanoma

Cancer

Lung

Collagen

Nano opto mechanical systems

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