5 June 2012 Monitoring the process of pulmonary melanoma metastasis using large area and label-free nonlinear optical microscopy
Author Affiliations +
Abstract
We performed large area nonlinear optical microscopy (NOM) for label-free monitoring of the process of pulmonary melanoma metastasis ex vivo with subcellular resolution in C57BL/6 mice. Multiphoton autofluorescence (MAF) and second harmonic generation (SHG) images of lung tissue are obtained in a volume of ∼2.2  mm  ×  2.2  mm  ×  30  μm. Qualitative differences in morphologic features and quantitative measurement of pathological lung tissues at different time points are characterized. We find that combined with morphological features, the quantitative parameters, such as the intensity ratio of MAF and SHG between pathological tissue and normal tissue and the MAF to SHG index versus depth clearly shows the tissue physiological changes during the process of pulmonary melanoma metastasis. Our results demonstrate that large area NOM succeeds in monitoring the process of pulmonary melanoma metastasis, which can provide a powerful tool for the research in tumor pathophysiology and therapy evaluation.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Daozhu Hua, Daozhu Hua, Shuhong Qi, Shuhong Qi, Hui Li, Hui Li, Zhihong Zhang, Zhihong Zhang, Ling Fu, Ling Fu, } "Monitoring the process of pulmonary melanoma metastasis using large area and label-free nonlinear optical microscopy," Journal of Biomedical Optics 17(6), 066002 (5 June 2012). https://doi.org/10.1117/1.JBO.17.6.066002 . Submission: Received: 5 December 2011; Accepted: 19 April 2012
Received: 5 December 2011; Accepted: 19 April 2012; Published: 5 June 2012
JOURNAL ARTICLE
6 PAGES


SHARE
Back to Top