4 June 2012 Scanning-fiber-based imaging method for tissue engineering
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A scanning-fiber-based method developed for imaging bioengineered tissue constructs such as synthetic carotid arteries is reported. Our approach is based on directly embedding one or more hollow-core silica fibers within the tissue scaffold to function as micro-imaging channels (MIC). The imaging process is carried out by translating and rotating an angle-polished fiber micro-mirror within the MIC to scan excitation light across the tissue scaffold. The locally emitted fluorescent signals are captured using an electron multiplying CCD camera and then mapped into fluorophore distributions according to fiber micro-mirror positions. Using an optical phantom composed of fluorescent microspheres, tissue scaffolds, and porcine skin, we demonstrated single-cell-level imaging resolution (20 to 30 μm) at an imaging depth that exceeds the photon transport mean free path by one order of magnitude. This result suggests that the imaging depth is no longer constrained by photon scattering, but rather by the requirement that the fluorophore signal overcomes the background “noise” generated by processes such as scaffold autofluorescence. Finally, we demonstrated the compatibility of our imaging method with tissue engineering by visualizing endothelial cells labeled with green fluorescent protein through a ∼500  μm thick and highly scattering electrospun scaffold.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Matthias C. Hofmann, Matthias C. Hofmann, Bryce M. Whited, Bryce M. Whited, Josh Mitchell, Josh Mitchell, William C. Vogt, William C. Vogt, Tracy L. Criswell, Tracy L. Criswell, Christopher G. Rylander, Christopher G. Rylander, Marissa N. Rylander, Marissa N. Rylander, Shay Soker, Shay Soker, Ge Wang, Ge Wang, Yong Xu, Yong Xu, } "Scanning-fiber-based imaging method for tissue engineering," Journal of Biomedical Optics 17(6), 066010 (4 June 2012). https://doi.org/10.1117/1.JBO.17.6.066010 . Submission: Received: 4 January 2012; Accepted: 17 April 2012
Received: 4 January 2012; Accepted: 17 April 2012; Published: 4 June 2012

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