5 June 2012 Quantitative photoacoustic microscopy of optical absorption coefficients from acoustic spectra in the optical diffusive regime
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Abstract
Photoacoustic (PA) microscopy (PAM) can image optical absorption contrast with ultrasonic spatial resolution in the optical diffusive regime. Conventionally, accurate quantification in PAM requires knowledge of the optical fluence attenuation, acoustic pressure attenuation, and detection bandwidth. We circumvent this requirement by quantifying the optical absorption coefficients from the acoustic spectra of PA signals acquired at multiple optical wavelengths. With the acoustic spectral method, the absorption coefficients of an oxygenated bovine blood phantom at 560, 565, 570, and 575 nm were quantified with errors of <3  %  . We also quantified the total hemoglobin concentration and hemoglobin oxygen saturation in a live mouse. Compared with the conventional amplitude method, the acoustic spectral method provides greater quantification accuracy in the optical diffusive regime. The limitations of the acoustic spectral method was also discussed.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Zijian Guo, Zijian Guo, Christopher P. Favazza, Christopher P. Favazza, Alejandro Garcia-Uribe, Alejandro Garcia-Uribe, Lihong V. Wang, Lihong V. Wang, } "Quantitative photoacoustic microscopy of optical absorption coefficients from acoustic spectra in the optical diffusive regime," Journal of Biomedical Optics 17(6), 066011 (5 June 2012). https://doi.org/10.1117/1.JBO.17.6.066011 . Submission: Received: 28 December 2011; Accepted: 12 April 2012
Received: 28 December 2011; Accepted: 12 April 2012; Published: 5 June 2012
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