Xiang Wen Britton Chance Ctr. for Biomedical Photonics (China) Dan Zhu Huazhong Univ. of Science and Technology (China) Steven L. Jacques Oregon Health & Science Univ. (United States) Valery V. Tuchin N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
The strong optical scattering of skin tissue makes it very difficult for optical coherence tomography (OCT) to achieve deep imaging in skin. Significant optical clearing of in vivo rat skin sites was achieved within 15 min by topical application of an optical clearing agent PEG-400, a chemical enhancer (thiazone or propanediol), and physical massage. Only when all three components were applied together could a 15 min treatment achieve a three fold increase in the OCT reflectance from a 300 μm depth and 31% enhancement in image depth Zthreshold.