6 June 2012 Label-free serum ribonucleic acid analysis for colorectal cancer detection by surface-enhanced Raman spectroscopy and multivariate analysis
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Abstract
Studies with circulating ribonucleic acid (RNA) not only provide new targets for cancer detection, but also open up the possibility of noninvasive gene expression profiling for cancer. In this paper, we developed a surface-enhanced Raman scattering (SERS), platform for detection and differentiation of serum RNAs of colorectal cancer. A novel three-dimensional (3-D), Ag nanofilm formed by dry MgSO4 aggregated silver nanoparticles, Ag NP, as the SERS-active substrate was presented to effectively enhance the RNA Raman signals. SERS measurements were performed on two groups of serum RNA samples. One group from patients, n  =  55 with pathologically diagnosed colorectal cancer and the other group from healthy controls, n  =  45. Tentative assignments of the Raman bands in the normalized SERS spectra demonstrated that there are differential expressions of cancer-related RNAs between the two groups. Linear discriminate analysis, based on principal component analysis, generated features can differentiate the colorectal cancer SERS spectra from normal SERS spectra with sensitivity of 89.1 percent and specificity of 95.6 percent. This exploratory study demonstrated great potential for developing serum RNA SERS analysis into a useful clinical tool for label-free, noninvasive screening and detection of colorectal cancers.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yanping Chen, Yanping Chen, Gang Chen, Gang Chen, Shangyuan Feng, Shangyuan Feng, Jianji Pan, Jianji Pan, Xiongwei Zheng, Xiongwei Zheng, Ying Su, Ying Su, Yan Chen, Yan Chen, Zufang Huang, Zufang Huang, Xiaoqian Lin, Xiaoqian Lin, Fenghua Lan, Fenghua Lan, Rong Chen, Rong Chen, Haishan Zeng, Haishan Zeng, } "Label-free serum ribonucleic acid analysis for colorectal cancer detection by surface-enhanced Raman spectroscopy and multivariate analysis," Journal of Biomedical Optics 17(6), 067003 (6 June 2012). https://doi.org/10.1117/1.JBO.17.6.067003 . Submission: Received: 31 December 2011; Accepted: 30 April 2012
Received: 31 December 2011; Accepted: 30 April 2012; Published: 6 June 2012
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