5 June 2012 Laser-induced modifications of gold nanoparticles and their cytotoxic effect
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As nanotechnology continues to develop, an assessment of nanoparticles’ toxicity becomes very crucial for biomedical applications. The current study examines the deleterious effects of pre-irradiated gold nanoparticles (GNPs) solutions on primary rat kidney cells (PRKCs). Spectroscopic and transmission electron microscopic studies demonstrated that exposure of 15 nm GNPs in size to pulsed laser caused a reduction both in optical density and mean particle diameter. GNPs showed an aggregation when added to the cell culture medium (DMEM). This aggregation was markedly decreased upon adding serum to the medium. Under our experimental conditions, trypan blue and MTT assays revealed no significant changes in cell viability when PRKCs were incubated with non-irradiated GNPs over a period of 72 h and up to 4 nM GNPs concentration. On the contrary, when cells were incubated with irradiated GNPs a significant reduction in PRKCs viability was revealed.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Shimaa Abdelhamid, Shimaa Abdelhamid, Hazem Saleh, Hazem Saleh, Mahmoud Abdelhamid, Mahmoud Abdelhamid, Adel Gohar, Adel Gohar, Tareq Youssef, Tareq Youssef, } "Laser-induced modifications of gold nanoparticles and their cytotoxic effect," Journal of Biomedical Optics 17(6), 068001 (5 June 2012). https://doi.org/10.1117/1.JBO.17.6.068001 . Submission: Received: 23 December 2011; Accepted: 24 April 2012
Received: 23 December 2011; Accepted: 24 April 2012; Published: 5 June 2012

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