16 May 2012 Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system
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Abstract
We report the tomographic imaging of a photodynamic therapy (PDT) photosensitizer, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) in vivo with time-domain fluorescence diffuse optical tomography (TD-FDOT). Simultaneous reconstruction of fluorescence yield and lifetime of HPPH was performed before and after PDT. The methodology was validated in phantom experiments, and depth-resolved in vivo imaging was achieved through simultaneous three-dimensional (3-D) mappings of fluorescence yield and lifetime contrasts. The tomographic images of a human head-and-neck xenograft in a mouse confirmed the preferential uptake and retention of HPPH by the tumor 24-h post-injection. HPPH-mediated PDT induced significant changes in fluorescence yield and lifetime. This pilot study demonstrates that TD-FDOT may be a good imaging modality for assessing photosensitizer distributions in deep tissue during PDT monitoring.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Weirong Mo, Weirong Mo, Daniel J. Rohrbach, Daniel J. Rohrbach, Ulas Sunar, Ulas Sunar, } "Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system," Journal of Biomedical Optics 17(7), 071306 (16 May 2012). https://doi.org/10.1117/1.JBO.17.7.071306 . Submission:
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