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3 July 2012 Deep ultraviolet resonant Raman imaging of a cell
Yasuaki Kumamoto, Atsushi Taguchi, Satoshi Kawata, Nicholas I. Smith
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Journal of Biomedical Optics, Vol. 17, Issue 7, 076001 (July 2012). https://doi.org/10.1117/1.JBO.17.7.076001
Abstract
We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of Raman microscopy as a tool to selectively probe nucleic acids in a cell with high sensitivity due to resonance.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Yasuaki Kumamoto, Atsushi Taguchi, Satoshi Kawata, and Nicholas I. Smith "Deep ultraviolet resonant Raman imaging of a cell," Journal of Biomedical Optics 17(7), 076001 (3 July 2012). https://doi.org/10.1117/1.JBO.17.7.076001
Published: 3 July 2012
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KEYWORDS
Raman spectroscopy
Deep ultraviolet
Microscopy
Molecules
Image processing
Raman scattering
Microscopes
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