Filter-based method for background removal in high-sensitivity wide-field-surface-enhanced Raman scattering imaging in vivo

Rupananda Mallia; Israel Veilleux; Brian C. Wilson; Patrick McVeigh

doi:10.1117/1.JBO.17.7.076017

13 July 2012 Filter-based method for background removal in high-sensitivity wide-field-surface-enhanced Raman scattering imaging in vivo

Rupananda Mallia, Israel Veilleux, Brian C. Wilson, Patrick McVeigh

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 7, 076017 (July 2012). https://doi.org/10.1117/1.JBO.17.7.076017

Abstract

As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluorescence background in vivo. SERS NPs have extremely narrow spectral peaks that do not overlap significantly with endogenous Raman signals. This can be exploited, using specific narrow-band filters, to image picomolar (pM) concentrations of NPs against a broad tissue autofluorescence background in wide-field mode, with short integration times that compare favorably with point-by-point mapping typically used in SERS imaging. This advance will facilitate the potential applications of SERS NPs as contrast agents in wide-field multiplexed biomarker-targeted imaging in vivo.

Citation Download Citation

Rupananda Mallia, Israel Veilleux, Brian C. Wilson, and Patrick McVeigh "Filter-based method for background removal in high-sensitivity wide-field-surface-enhanced Raman scattering imaging in vivo," Journal of Biomedical Optics 17(7), 076017 (13 July 2012). https://doi.org/10.1117/1.JBO.17.7.076017

Published: 13 July 2012

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY