24 July 2012 Visualization of the solubilization process of the plasma membrane of a living cell by waveguide evanescent field fluorescence microscopy
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Abstract
Waveguide evanescent field fluorescence microscopy (WEFF) is a novel microscopy technology that allows imaging of a cell's plasma membrane in the vicinity of a glass substrate with high axial resolution, low background and little photobleaching. Time-lapse imaging can be performed to investigate changes in cell morphology in the presence or absence of chemical agents. WEFF microscopy provides a method to investigate plasma membranes of living cells and allows a comparison to simplified model membranes immobilized on planar substrates. The interaction of the nonionic detergent Triton X-100 with plasma membranes of osteoblasts in an aqueous environment was investigated. Solubilization of the membranes very close to the waveguide surface was visualized and related to the three-stage solubilisation model proposed for liposomes and supported lipid bilayers. Findings for the plasma membranes of cells are in excellent agreement with results reported for these artificial model systems.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Abdollah Hassanzadeh, Abdollah Hassanzadeh, Heung K. Ma, Heung K. Ma, Silvia Mittler, Silvia Mittler, S. Jeffrey Dixon, S. Jeffrey Dixon, } "Visualization of the solubilization process of the plasma membrane of a living cell by waveguide evanescent field fluorescence microscopy," Journal of Biomedical Optics 17(7), 076025 (24 July 2012). https://doi.org/10.1117/1.JBO.17.7.076025 . Submission:
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