Imaging-guided two-photon excitation-emission-matrix measurements of human skin tissues

Yingqiu Yu; Anthony M. Lee; Hequn Wang; Jianhua Zhao; Harvey Lui M.D.; Haishan Zeng; Shuo Tang

doi:10.1117/1.JBO.17.7.077004

6 July 2012 Imaging-guided two-photon excitation-emission-matrix measurements of human skin tissues

Yingqiu Yu, Anthony M. Lee, Hequn Wang, Jianhua Zhao, Harvey Lui M.D., Haishan Zeng, Shuo Tang

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 7, 077004 (July 2012). https://doi.org/10.1117/1.JBO.17.7.077004

Abstract

There are increased interests on using multiphoton imaging and spectroscopy for skin tissue characterization and diagnosis. However, most studies have been done with just a few excitation wavelengths. Our objective is to perform a systematic study of the two-photon fluorescence (TPF) properties of skin fluorophores, normal skin, and diseased skin tissues. A nonlinear excitation-emission-matrix (EEM) spectroscopy system with multiphoton imaging guidance was constructed. A tunable femtosecond laser was used to vary excitation wavelengths from 730 to 920 nm for EEM data acquisition. EEM measurements were performed on excised fresh normal skin tissues, seborrheic keratosis tissue samples, and skin fluorophores including: NADH, FAD, keratin, melanin, collagen, and elastin. We found that in the stratum corneum and upper epidermis of normal skin, the cells have large sizes and the TPF originates from keratin. In the lower epidermis, cells are smaller and TPF is dominated by NADH contributions. In the dermis, TPF is dominated by elastin components. The depth resolved EEM measurements also demonstrated that keratin structure has intruded into the middle sublayers of the epidermal part of the seborrheic keratosis lesion. These results suggest that the imaging guided TPF EEM spectroscopy provides useful information for the development of multiphoton clinical devices for skin disease diagnosis.

Citation Download Citation

Yingqiu Yu, Anthony M. Lee, Hequn Wang, Jianhua Zhao, Harvey Lui M.D., Haishan Zeng, and Shuo Tang "Imaging-guided two-photon excitation-emission-matrix measurements of human skin tissues," Journal of Biomedical Optics 17(7), 077004 (6 July 2012). https://doi.org/10.1117/1.JBO.17.7.077004

Published: 6 July 2012

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY