Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source

Cuixia Dai; Xiaojing Liu; Shuliang Jiao

doi:10.1117/1.JBO.17.8.080502

13 July 2012 Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source

Cuixia Dai, Xiaojing Liu, Shuliang Jiao

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Journal of Biomedical Optics, Vol. 17, Issue 8, 080502 (July 2012). https://doi.org/10.1117/1.JBO.17.8.080502

Abstract

We have accomplished simultaneous spectral domain optical coherence tomography (SD-OCT) and autofluorescence (AF) microscopy with a broadband light source centered at 415 nm. The light source was provided by frequency-doubling of an ultra-fast broadband Ti:Sapphire laser. With a bandwidth of 8 nm, the visible SD-OCT achieved a depth resolution of ∼ 12 μm. Since the two imaging modalities are provided by the same group of photons, their images are intrinsically registered. The dual-modal system is capable of providing OCT imaging and molecular contrasts simultaneously. The imaging system was tested on imaging biological samples ex vivo and in vivo.

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Cuixia Dai, Xiaojing Liu, and Shuliang Jiao "Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source," Journal of Biomedical Optics 17(8), 080502 (13 July 2012). https://doi.org/10.1117/1.JBO.17.8.080502

Published: 13 July 2012

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