Mosaic acquisition and processing for optical-resolution photoacoustic microscopy

Peng Shao; Wei Shi; Ryan K. Chee; Roger J. Zemp

doi:10.1117/1.JBO.17.8.080503

19 July 2012 Mosaic acquisition and processing for optical-resolution photoacoustic microscopy

Peng Shao, Wei Shi, Ryan K. Chee, Roger J. Zemp

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 8, 080503 (July 2012). https://doi.org/10.1117/1.JBO.17.8.080503

Abstract

In optical-resolution photo-acoustic microscopy (OR-PAM), data acquisition time is limited by both laser pulse repetition rate (PRR) and scanning speed. Optical-scanning offers high speed, but limited, field of view determined by ultrasound transducer sensitivity. In this paper, we propose a hybrid optical and mechanical-scanning OR-PAM system with mosaic data acquisition and processing. The system employs fast-scanning mirrors and a diode-pumped, nanosecond-pulsed, Ytterbium-doped, 532-nm fiber laser with PRR up to 600 kHz. Data from a sequence of image mosaic patches is acquired systematically, at predetermined mechanical scanning locations, with optical scanning. After all imaging locations are covered, a large panoramic scene is generated by stitching the mosaic patches together. Our proposed system is proven to be at least 20 times faster than previous reported OR-PAM systems.

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Peng Shao, Wei Shi, Ryan K. Chee, and Roger J. Zemp "Mosaic acquisition and processing for optical-resolution photoacoustic microscopy," Journal of Biomedical Optics 17(8), 080503 (19 July 2012). https://doi.org/10.1117/1.JBO.17.8.080503

Published: 19 July 2012

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