Dynamic focusing of light into thick biological tissue is desired for noninvasive optical imaging, diagnostics, manipulation, and therapy. However, multiple light scattering in biological tissue limits the focusing of ballistic photons to shallow depths of about one transport mean free path. To solve this problem, Xu et al. developed a technique named time-reversed ultrasonically encoded (TRUE) optical focusing.1 Using a focused ultrasound (US) beam, the technique spectrally tags the diffuse coherent light inside a scattering medium. Optical focusing into the medium is achieved by selectively phase conjugating, or time-reversing, only the tagged light, using a photorefractive (PR) crystal as a phase conjugate mirror (PCM). Recently, Lai et al. implemented a TRUE optical focusing system in reflection mode,2 which demonstrated a round-trip optical penetration of 80 scattering mean free paths.
These systems used a photorefractive (BSO) crystal as a PCM. However, the transverse dimension of the BSO crystal, like all other inorganic PR crystals, is at most a few centimeters,3 which limits the area for diffuse light collection. Furthermore, BSO has other inferior figures of merit, such as its hologram persistency, its PR response time, and its holographic diffraction efficiency. Photorefractive polymers (PRP) show exciting potential to overcome these limitations. Recent studies have demonstrated PRPs with large active areas and high diffraction efficiencies3 (close to 100%). Further, PRPs can be tuned to have long hologram persistency,4 or fast response time ().5 Thus, PRPs can improve TRUE optical focusing by increasing focused energy or accelerating focusing. In this letter, we report the first study using a PRP as the PCM in TRUE optical focusing to enhance focused energy.
The PRP, supplied by Nitto Denko Technical, has the same polymer composite as the one reported in Ref. 4, and consists of a 100-μm-thick polymer film sandwiched between two indium-tin-oxide (ITO) coated glass electrodes. The working wavelength of our polymer is set around our laser wavelength (532 nm). The inset of Fig. 1(a) compares the dimensions of the PRP and BSO crystal used in the previous and current experiments. While the BSO crystal has an active area of , the PRP has an active area of , yielding an etendue as large with the same collection geometry.
Figure 1(a) shows the TRUE optical focusing experimental setup, which is similar to that of Ref. 1. In this study, the diameter of the sample beam incident on the scattering sample was 3 mm, and the diameters of both the reference beam and readout beam were expanded to 30 mm. The PCM was either a BSO crystal or a PRP. When a BSO crystal was used, it was positioned so that its optical surface normal almost bisected the angle () between the incident reference beam and the diffused sample beam (hereafter called “scattered ”). To enhance the phase conjugation efficiency,6 a 2.1-kHz square-waveform voltage with a peak-to-peak amplitude of 8 kV was applied across the optical surface, yielding an electric field of . When a PRP was used as the PCM, its surface normal was horizontally tilted from the propagation direction of the reference beam by , as shown in Fig. 1(b). A 4 kV DC voltage was applied between the polymer’s front and back surfaces (the corresponding electric field was ) to enable its PR performance. In the tested tilted geometry, the etendue of the PRP is estimated to be using its acceptance angle from Ref. 7.
A hologram was recorded then reconstructed in the PR material. In the holographic recording phase, was switched on, and after diffusively propagating through the sample, was collected to illuminate the PR material. A focused continuous-wave US beam, with a focal pressure of 1 MPa, focal width of , and focal zone length of , was emitted from a 2 MHz transducer (Sonic Concepts, H106), modulating the diffuse light in the sample. The frequency of was set to 2 MHz above that of . Therefore, only the ultrasonically down-modulated light from the sample formed a stationary interference pattern with , which was recorded in the PR material as a volume hologram. Once was switched off after 800 ms holographic recording, shutters S1 and S2 were opened to start the readout phase. Then a 50-ms-long * pulse—a counter-propagating phase-conjugated version of —was switched on to read the hologram, which generated , a time-reversed (TR) copy of the tagged light, to achieve optical focusing. After transmitting through the sample a second time, was detected by a silicon photodiode (PD1) as the TRUE signal. Under these experimental conditions, the holographic diffraction efficiency of our PRP reached its steady state value after , which agreed with the rise time estimated by its supplier. In the PRP experiments, we waited for 30 s after changing any parameters to let the recorded hologram reach steady state before taking any measurements.
To evaluate the performances of the system based on either the PRP or BSO crystal as a PCM, we acquired TRUE signals using a 1-cm-thick gelatin-intralipid sample having a reduced scattering coefficient . Figure 2(a) shows the measured peak intensity of the TRUE signal versus the intensity ratio of scattered to in both scenarios. We fixed the and intensities at and , respectively, and increased the intensity while monitoring it with PD2. When we changed the PCM, we adjusted mirrors M3 and M4 in Fig. 1(b) to maximize the TR signal at PD1 before taking any measurement. The TRUE signal in this experiment did not show a sharp signal rise accompanied with a fast decay, as in Ref. 2. This is because we used a lower intensity here compared with that in Ref. 2, and the dependence of the TRUE waveform on the intensity was reported previously.8 It can be seen that the PRP generated signal is stronger than the BSO crystal, thus enabling stronger TRUE focusing.
Seen in Fig. 2(a) is the linear relationship between the TRUE signal and the intensity ratio of scattered to . To understand this linear relationship, we can describe the interferogram as the intensity averaged over the response time of the PRP:1) is the intensity fringe responsible for the hologram recording, and the first two terms are the background. Because is much stronger than , the contribution to the background is negligible. Therefore, the fringe contrast is proportional to the amplitude ratio . From the theory of PR materials, it is known that the holographic diffraction efficiency is proportional to the squared contrast (or modulation) of the interference fringe that engraves the hologram,9 i.e., . Thus, the TRUE signal linearly increased with or the intensity while was fixed.
Figure 2(b) shows the temporal profiles of TRUE signals generated by PRP and BSO, respectively. In both cases, the power of was 280 mW, and the corresponding intensity ratio of scattered to was 0.14. In addition to the increase in the peak signal intensity, the TRUE signal generated by the PRP lasted longer than that of the BSO crystal as quantified by the time to 50% drop from the maximum. The focused optical energy generated by the PRP was increased by at least 40-fold over the BSO systems reported earlier, as computed by the areas under the curves.
To demonstrate PRP’s improved penetration capability, we imaged a phantom sample using TRUE optical focusing. The phantom was a gelatin-intralipid mixture, having a thickness of 1 cm and a reduced scattering coefficient . Embedded in the middle plane of the sample were two optical absorbers Obj1 and Obj2, with thicknesses of 0.5 mm and 0.4 mm along the axis, respectively, made by adding black India ink to the background solution before it gelled. The absorption coefficients () of the turbid background and the optical absorbers were measured to be and , respectively. Figure 3(a) shows a photo of the imaging plane containing the two absorbers. The power of was 250 mW, spread over an area of on the incident sample surface, whereas the intensity of was , and the intensity of was . The US transducer was aligned so that its focal zone intersected with the optical axis along . The US beam scanned along across the optical absorbers with a step size of 0.32 mm. The TRUE signal was obtained by subtracting the averaged (32 times) PD1 signal when the US was off from the PD1 signal when the US was on. Figure 3(b) shows the normalized TRUE signal versus . As a comparison, we also plotted the normalized distributions of the direct-current (DC) signal, defined as scattered intensity acquired without US at the PRP position, and the TR direct-current (TRDC) signal acquired as the TR signal without US when and share the same frequency. As shown in Fig. 3(b), the TRUE signal drops when the US focal zone intersects the two optical absorbers at and 14 mm, respectively. By contrast, the DC and TRDC images do not reveal the two absorbers clearly as a result of the strong light scattering in the sample. The optical thickness of the sample is is the geometric thickness of the sample) or , where the anisotropic factor .10 The sample thickness presented here far exceeds the maximum penetration previously reported in TRUE imaging experiments,1,2,11 which illustrates the enhanced energy focused by the PRP.
The PRP used in this study does have drawbacks: signal instability over a long time (), a relatively low optical damage threshold (), and a slow response time (). Also, in certain applications that require rapid scanning of the focal spot, long persistency of the hologram may become undesirable. However, monitoring of the two-wave mixing gain as a reference signal to normalize the measured TRUE signal may compensate for the signal fluctuation. Moreover, maintaining the beam intensity below the PRP damage threshold while expanding the beam size of and can further increase the focused light intensity. For future in vivo experiments, it is necessary to shorten the holographic rise time to within the speckle correlation time of tissue (). Also, to compensate for the slow rise time, we used a continuous-wave US beam in this work, which deteriorates the focal resolution along the acoustic axis ( axis). In tackling these problems, one may seek to improve the rise time by increasing the intensity without exceeding the PRP’s optical damage threshold. On the other hand, combinations of a pulsed laser and fast PRPs may potentially enable in vivo experiments by providing a fast response and a large active area,5 while improving the acoustic axial resolution by the use of short synchronized US pulses. It was also reported that applying a higher voltage () on the polymer accelerates PR response, and thus controls the rise time and persistency to some extent.4 For tissue penetration, wavelengths in the optical window (approximately 600 to 1300 nm) are more suitable. By choosing the appropriate wavelength sensitive composites, PRPs can be tuned to work within the desired wavelength range.12 Thus, the disadvantages of the current PRP are likely to be overcome, and its efficient light focusing ability is highly attractive. For example, while the penetration thickness of the one-dimensional (1-D) image presented here was 120, the maximum penetration thickness of a phantom from which we observed a TRUE signal was 200 (data not shown).
In summary, using a PRP considerably enhanced the focused energy in TRUE optical focusing. We demonstrated a 1-D imaging result with TRUE optical focusing at an optical thickness of 120 scattering mean free paths. Further improvement can be expected by increasing the hologram area and using a PRP with faster response time.
We would like to thank Nitto Denko Technical@Oceanside, C.A. for helpful discussions and providing the PRP for this research. This work was sponsored by the National Academies Keck Futures Initiative grant IS 13. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
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