Multiwavelength time-resolved detection of fluorescence during the inflow of indocyanine green into the adult's brain

Anna Gerega; Daniel Milej; Marcin Botwicz; Norbert Zolek; Michal Kacprzak; Beata Toczylowska; Roman Maniewski; Adam Liebert; Wojciech Weigl; Ewa Mayzner-Zawadzka; Wojciech Wierzejski

doi:10.1117/1.JBO.17.8.087001

2 August 2012 Multiwavelength time-resolved detection of fluorescence during the inflow of indocyanine green into the adult's brain

Anna Gerega; Daniel Milej; Marcin Botwicz; Norbert Zolek; Michal Kacprzak; Beata Toczylowska; Roman Maniewski; Adam Liebert; Wojciech Weigl; Ewa Mayzner-Zawadzka; Wojciech Wierzejski;

Author Affiliations +

J. of Biomedical Optics, 17(8), 087001 (2012). https://doi.org/10.1117/1.JBO.17.8.087001

Abstract

Optical technique based on diffuse reflectance measurement combined with indocyanine green (ICG) bolus tracking is extensively tested as a method for clinical assessment of brain perfusion in adults at the bedside. Methodology of multiwavelength and time-resolved detection of fluorescence light excited in the ICG is presented and advantages of measurements at multiple wavelengths are discussed. Measurements were carried out: 1. on a physical homogeneous phantom to study the concentration dependence of the fluorescence signal, 2. on the phantom to simulate the dynamic inflow of ICG at different depths, and 3. in vivo on surface of the human head. Pattern of inflow and washout of ICG in the head of healthy volunteers after intravenous injection of the dye was observed for the first time with time-resolved instrumentation at multiple emission wavelengths. The multiwavelength detection of fluorescence signal confirms that at longer emission wavelengths, probability of reabsorption of the fluorescence light by the dye itself is reduced. Considering different light penetration depths at different wavelengths, and the pronounced reabsorption at longer wavelengths, the time-resolved multiwavelength technique may be useful in signal decomposition, leading to evaluation of extra- and intracerebral components of the measured signals.

Citation Download Citation

Anna Gerega, Daniel Milej, Marcin Botwicz, Norbert Zolek, Michal Kacprzak, Beata Toczylowska, Roman Maniewski, Adam Liebert, Wojciech Weigl, Ewa Mayzner-Zawadzka, Wojciech Wierzejski, "Multiwavelength time-resolved detection of fluorescence during the inflow of indocyanine green into the adult's brain," Journal of Biomedical Optics 17(8), 087001 (2 August 2012). https://doi.org/10.1117/1.JBO.17.8.087001 . Submission:

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $20.00

Non-members: $25.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years