CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 17 > Issue 9 > Article
Open Access
6 September 2012 Noninvasive Raman spectroscopy of rat tibiae: approach to in vivo assessment of bone quality
Paul I. Okagbare, Michael D. Morris, Dana Begun, Steven A. Goldstein, Mary Tecklenburg
Author Affiliations +
Journal of Biomedical Optics, Vol. 17, Issue 9, 090502 (September 2012). https://doi.org/10.1117/1.JBO.17.9.090502
Abstract
We report on in vivo noninvasive Raman spectroscopy of rat tibiae using robust fiber-optic Raman probes and holders designed for transcutaneous Raman measurements in small animals. The configuration allows placement of multiple fibers around a rat leg, maintaining contact with the skin. Bone Raman data are presented for three regions of the rat tibia diaphysis with different thicknesses of overlying soft tissue. The ability to perform in vivo noninvasive Raman measurement and evaluation of subtle changes in bone composition is demonstrated with rat leg phantoms in which the tibia has carbonated hydroxylapatite, with different carbonate contents. Our data provide proof of the principle that small changes in bone composition can be monitored through soft tissue at anatomical sites of interest in biomedical studies.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
Citation Download Citation
Paul I. Okagbare, Michael D. Morris, Dana Begun, Steven A. Goldstein, and Mary Tecklenburg "Noninvasive Raman spectroscopy of rat tibiae: approach to in vivo assessment of bone quality," Journal of Biomedical Optics 17(9), 090502 (6 September 2012). https://doi.org/10.1117/1.JBO.17.9.090502
Published: 6 September 2012
JOURNAL ARTICLE
 3 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 38 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Raman spectroscopy
Bone
Optical fibers
Tissues
In vivo imaging
Carbonates
Tissue optics
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top