1 September 2012 Impact of the optical depth of field on cytogenetic image quality
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Abstract
In digital pathology, clinical specimen slides are converted into digital images by microscopic image scanners. Since random vibration and mechanical drifting are unavoidable on even high-precision moving stages, the optical depth of field (DOF) of microscopic systems may affect image quality, in particular when using an objective lens with high magnification power. The DOF of a microscopic system was theoretically analyzed and experimentally validated using standard resolution targets under
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yuchen Qiu, Yuhua Li, Hong Liu, Xiaodong Chen, Bin Zheng, Shibo Li, Wei R. Chen, "Impact of the optical depth of field on cytogenetic image quality," Journal of Biomedical Optics 17(9), 096017 (1 September 2012). https://doi.org/10.1117/1.JBO.17.9.096017 . Submission:
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