15 July 2013 Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells
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Abstract
An optical lensless configuration for a remote noncontact measuring of mechanical contractions of a vast number of cardiac myocytes is proposed. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (∼1000 cells) and to extract the statistical data of their movement at once. The presented experimental results also include investigation of the effect of isoproteranol on cell contraction process.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Mark Golberg, Mark Golberg, Dror Fixler, Dror Fixler, Asher Shainberg, Asher Shainberg, Sharon Zlochiver, Sharon Zlochiver, Vicente Micó, Vicente Micó, Javier Garcia, Javier Garcia, Yevgeny Beiderman, Yevgeny Beiderman, Zeev Zalevsky, Zeev Zalevsky, } "Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells," Journal of Biomedical Optics 18(10), 101310 (15 July 2013). https://doi.org/10.1117/1.JBO.18.10.101310 . Submission:
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