Multimodal tissue imaging: using coregistered optical tomography data to estimate tissue autofluorescence intensity change due to scattering and absorption by neoplastic epithelial cells

Hamid Pahlevaninezhad; Ivana Cecic; Anthony M. Lee; Alastair H. Kyle; Stephen Lam M.D.; Calum MacAulay; Pierre M. Lane

doi:10.1117/1.JBO.18.10.106007

9 October 2013 Multimodal tissue imaging: using coregistered optical tomography data to estimate tissue autofluorescence intensity change due to scattering and absorption by neoplastic epithelial cells

Hamid Pahlevaninezhad, Ivana Cecic, Anthony M. Lee, Alastair H. Kyle, Stephen Lam M.D., Calum MacAulay, Pierre M. Lane

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Journal of Biomedical Optics, Vol. 18, Issue 10, 106007 (October 2013). https://doi.org/10.1117/1.JBO.18.10.106007

Abstract

Autofluorescence (AF) imaging provides valuable information about the structural and chemical states of tissue that can be used for early cancer detection. Optical scattering and absorption of excitation and emission light by the epithelium can significantly affect observed tissue AF intensity. Determining the effect of epithelial attenuation on the AF intensity could lead to a more accurate interpretation of AF intensity. We propose to use optical coherence tomography coregistered with AF imaging to characterize the AF attenuation due to the epithelium. We present imaging results from three vital tissue models, each consisting of a three-dimensional tissue culture grown from one of three epithelial cell lines (HCT116, OVCAR8, and MCF7) and immobilized on a fluorescence substrate. The AF loss profiles in the tissue layer show two different regimes, each approximately linearly decreasing with thickness. For thin cell cultures (<300 μm ), the AF signal changes as AF(t)/AF(0)=1−1.3[i]t ([b]t is the thickness in millimeter). For thick cell cultures (<400 μm ), the AF loss profiles have different intercepts but similar slopes. The data presented here can be used to estimate AF loss due to a change in the epithelial layer thickness and potentially to reduce AF bronchoscopy false positives due to inflammation and non-neoplastic epithelial thickening.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Hamid Pahlevaninezhad, Ivana Cecic, Anthony M. Lee, Alastair H. Kyle, Stephen Lam M.D., Calum MacAulay, and Pierre M. Lane "Multimodal tissue imaging: using coregistered optical tomography data to estimate tissue autofluorescence intensity change due to scattering and absorption by neoplastic epithelial cells," Journal of Biomedical Optics 18(10), 106007 (9 October 2013). https://doi.org/10.1117/1.JBO.18.10.106007

Published: 9 October 2013

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