Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues

Mohammed Mesradi; Aurelie Genoux; Vesna Cuplov; Darine Abi-Haidar; Sebastien Jan; Irene Buvat; Frederic Pain

doi:10.1117/1.JBO.18.11.117010

18 November 2013 Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues

Mohammed Mesradi, Aurelie Genoux, Vesna Cuplov, Darine Abi-Haidar, Sebastien Jan, Irene Buvat, Frederic Pain

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Journal of Biomedical Optics, Vol. 18, Issue 11, 117010 (November 2013). https://doi.org/10.1117/1.JBO.18.11.117010

Abstract

Optical properties of fresh and frozen tissues of rat heart, kidney, brain, liver, and muscle were measured in the 450- to 700-nm range. The total reflectance and transmittance were measured using a well-calibrated integral sphere set-up. Absorption coefficient μ_a and reduced scattering coefficient μ′_s were derived from the experimental measurements using the inverse adding doubling technique. The influence of cryogenic processing on optical properties was studied. Interindividual and intraindividual variations were assessed. These new data aim at filling the lack of validated optical properties in the visible range especially in the blue-green region of particular interest for fluorescence and optogenetics preclinical studies. Furthermore, we provide a unique comparison of the optical properties of different organs obtained using the same measurement set-up for fresh and frozen tissues as well as an estimate of the intraindividual and interindividual variability.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Mohammed Mesradi, Aurelie Genoux, Vesna Cuplov, Darine Abi-Haidar, Sebastien Jan, Irene Buvat, and Frederic Pain "Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues," Journal of Biomedical Optics 18(11), 117010 (18 November 2013). https://doi.org/10.1117/1.JBO.18.11.117010

Published: 18 November 2013

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