Nanosecond ratio imaging of redox states in tumor cell spheroids using light sheet-based fluorescence microscopy

Sarah Schickinger; Thomas Bruns; Rainer Wittig; Petra Weber; Michael Wagner; Herbert Schneckenburger

doi:10.1117/1.JBO.18.12.126007

16 December 2013 Nanosecond ratio imaging of redox states in tumor cell spheroids using light sheet-based fluorescence microscopy

Sarah Schickinger, Thomas Bruns, Rainer Wittig, Petra Weber, Michael Wagner, Herbert Schneckenburger

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Journal of Biomedical Optics, Vol. 18, Issue 12, 126007 (December 2013). https://doi.org/10.1117/1.JBO.18.12.126007

Abstract

A new concept of three-dimensional imaging of tumor cell spheroids by light sheet-based fluorescence microscopy and nanosecond ratio imaging is described. Due to its low light dose and alternative excitation by two laser wavelengths (391 and 470 nm), this method maintains cell viability and permits recording of real-time kinetics. A genetically encoded sensor permits measurement of the redox state of glutathione and visualization of the impact of oxygen radicals. The pharmaceutically relevant system is tested upon addition of an oxidizing agent (H₂O₂ ), as well as upon addition of the apoptosis-inducing agent staurosporine.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Sarah Schickinger, Thomas Bruns, Rainer Wittig, Petra Weber, Michael Wagner, and Herbert Schneckenburger "Nanosecond ratio imaging of redox states in tumor cell spheroids using light sheet-based fluorescence microscopy," Journal of Biomedical Optics 18(12), 126007 (16 December 2013). https://doi.org/10.1117/1.JBO.18.12.126007

Published: 16 December 2013

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