Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models

Ali A. Maawy; Yukihiko Hiroshima; Sharmeela Kaushal; George A. Luiken; Robert M. Hoffman; Michael Bouvet

doi:10.1117/1.JBO.18.12.126016

19 December 2013 Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models

Ali A. Maawy, Yukihiko Hiroshima, Sharmeela Kaushal, George A. Luiken, Robert M. Hoffman, Michael Bouvet

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Journal of Biomedical Optics, Vol. 18, Issue 12, 126016 (December 2013). https://doi.org/10.1117/1.JBO.18.12.126016

Abstract

The aim of this study was to evaluate a set of visible and near-infrared dyes conjugated to a tumor-specific chimeric antibody for high-resolution tumor imaging in orthotopic models of pancreatic cancer. BxPC-3 human pancreatic cancer was orthotopically implanted into pancreata of nude mice. Mice received a single intravenous injection of a chimeric anti-carcinoembryonic antigen antibody conjugated to one of the following fluorophores: 488-nm group (Alexa Fluor 488 or DyLight 488); 550-nm group (Alexa Fluor 555 or DyLight 550); 650-nm group (Alexa Fluor 660 or DyLight 650), or the 750-nm group (Alexa Fluor 750 or DyLight 755). After 24 h, the Olympus OV100 small-animal imaging system was used for noninvasive and intravital fluorescence imaging of mice. Dyes were compared with respect to depth of imaging, resolution, tumor-to-background ratio (TBR), photobleaching, and hemoglobin quenching. The longer wavelength dyes had increased depth of penetration and ability to detect the smallest tumor deposits and provided the highest TBRs, resistance to hemoglobin quenching, and specificity. The shorter wavelength dyes were more photostable. This study showed unique advantages of each dye for specific cancer imaging in a clinically relevant orthotopic model.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Ali A. Maawy, Yukihiko Hiroshima, Sharmeela Kaushal, George A. Luiken, Robert M. Hoffman, and Michael Bouvet "Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models," Journal of Biomedical Optics 18(12), 126016 (19 December 2013). https://doi.org/10.1117/1.JBO.18.12.126016

Published: 19 December 2013

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