23 December 2013 Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors
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Abstract
Several studies have shown that low-level laser irradiation (LLLI) has beneficial effects on bone regeneration. The objective of this study was to examine the in vitro effects of LLLI on proliferation and differentiation of a human osteoblast-like cell line (Saos-2 cell line). Cultured cells were exposed to different doses of LLLI with a semiconductor diode laser (659 nm; 10 mW power output). The effects of laser on proliferation were assessed daily up to seven days of culture in cells irradiated once or for three consecutive days with laser doses of 1 or 3  J/cm 2 . The obtained results showed that laser stimulation enhances the proliferation potential of Saos-2 cells without changing their telomerase pattern or morphological characteristics. The effects on cell differentiation were assessed after three consecutive laser irradiation treatments in the presence or absence of osteo-inductive factors on day 14. Enhanced secretion of proteins specific for differentiation toward bone as well as calcium deposition and alkaline phosphatase activity were observed in irradiated cells cultured in a medium not supplemented with osteogenic factors. Taken together these findings indicate that laser treatment enhances the in vitro proliferation of Saos-2 cells, and also influences their osteogenic maturation, which suggest it is a helpful application for bone tissue regeneration.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Nora Bloise, Nora Bloise, Gabriele Ceccarelli, Gabriele Ceccarelli, Paolo Minzioni, Paolo Minzioni, Marco Vercellino, Marco Vercellino, Laura Benedetti, Laura Benedetti, Maria Gabriella C. De Angelis, Maria Gabriella C. De Angelis, Marcello Imbriani, Marcello Imbriani, Livia Visai, Livia Visai, } "Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors," Journal of Biomedical Optics 18(12), 128006 (23 December 2013). https://doi.org/10.1117/1.JBO.18.12.128006 . Submission:
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