1 February 2013 Single-cell photoacoustic thermometry
Author Affiliations +
J. of Biomedical Optics, 18(2), 026003 (2013). doi:10.1117/1.JBO.18.2.026003
Abstract
A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Liang Gao, Lidai Wang, Chiye Li, Yan Liu, Haixin Ke, Chi Zhang, Lihong V. Wang, "Single-cell photoacoustic thermometry," Journal of Biomedical Optics 18(2), 026003 (1 February 2013). http://dx.doi.org/10.1117/1.JBO.18.2.026003
JOURNAL ARTICLE
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KEYWORDS
Photoacoustic spectroscopy

Temperature metrology

Thermometry

Calibration

Particles

Pulsed laser operation

Acoustics

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