1 February 2013 Single-cell photoacoustic thermometry
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Abstract
A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Liang Gao, Liang Gao, Lidai Wang, Lidai Wang, Chiye Li, Chiye Li, Yan Liu, Yan Liu, Haixin Ke, Haixin Ke, Chi Zhang, Chi Zhang, Lihong V. Wang, Lihong V. Wang, } "Single-cell photoacoustic thermometry," Journal of Biomedical Optics 18(2), 026003 (1 February 2013). https://doi.org/10.1117/1.JBO.18.2.026003 . Submission:
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