Liang Gao Washington Univ. in St. Louis (United States) Lidai Wang Washington Univ. School of Medicine in St. Louis (United States) Chiye Li,Yan Liu,Haixin Ke,Chi Zhang,Lihong V. Wang Washington Univ. in St. Louis (United States)
A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.