Reflection-mode multifocal optical-resolution photoacoustic microscopy

Guo Li; Konstantin I. Maslov; Lihong V. Wang

doi:10.1117/1.JBO.18.3.030501

27 February 2013 Reflection-mode multifocal optical-resolution photoacoustic microscopy

Guo Li, Konstantin I. Maslov, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 18, Issue 3, 030501 (February 2013). https://doi.org/10.1117/1.JBO.18.3.030501

Abstract

Compared with single-focus optical-resolution photoacoustic microscopy (OR-PAM), multifocal OR-PAM utilizes both multifocal optical illumination and an ultrasonic array transducer, significantly increasing the imaging speed. A reflection-mode multifocal OR-PAM system based on a microlens array that provides multiple foci as well as an ultrasonic array transducer that receives the excited photoacoustic waves from all foci simultaneously is presented. Using a customized microprism to reflect the incident laser beam to the microlens array, the multiple optical foci are aligned confocally with the focal zone of the ultrasonic array transducer. Experiments show the reflection-mode multifocal OR-PAM is capable of imaging microvessels in vivo, and it can image a 6×5×2.5 mm³ volume at 16 μm lateral resolution in ∼2.5 min , which was limited by the signal multiplexing ratio and laser pulse repetition rate.

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Guo Li, Konstantin I. Maslov, and Lihong V. Wang "Reflection-mode multifocal optical-resolution photoacoustic microscopy," Journal of Biomedical Optics 18(3), 030501 (27 February 2013). https://doi.org/10.1117/1.JBO.18.3.030501

Published: 27 February 2013

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