22 November 2012 Analysis of collagen fiber domain organization by Fourier second harmonic generation microscopy
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J. of Biomedical Optics, 18(3), 031105 (2012). doi:10.1117/1.JBO.18.3.031105
We present an automated and systematic two-dimensional discrete Fourier transform (2D-FFT) approach to analyze collagen fiber organization through the use of second harmonic generation (SHG) microscopy. Average orientations of individual domains and Ising-like order parameters introduced to characterize the correlation between orientations of adjacent domains may be used to quantitatively characterize fibrous tissues. Our approach was applied to analyze tissues including rat tail tendon, mouse skin, bovine corneas, and human corneas. We also show that collagen fiber organization in normal and keratokonus human corneas may be distinguished. The current approach may be used for the quantitative differentiation of SHG collagen fiber morphology in different tissues and may be applied for diagnostic purposes.
© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Ara Ghazaryan, Halley F. Tsai, Gor Hayrapetyan, Wei-Liang Chen, Yang-Fang Chen, Myung-Yung Jeong, Chang-Seok Kim, Shean-Jen Chen, Chen-Yuan Dong, "Analysis of collagen fiber domain organization by Fourier second harmonic generation microscopy," Journal of Biomedical Optics 18(3), 031105 (22 November 2012). https://doi.org/10.1117/1.JBO.18.3.031105


Second-harmonic generation



Autoregressive models

Harmonic generation


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